CA9 Monoklonaler Antikörper

CA9 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

1G4G5

Rezensionen (1)

Kat-Nr. : 66243-1-Ig

Synonyme

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

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Galerie der Validierungsdaten

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  WB analysis of U-87 MG using 66243-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig stomach using 66243-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66243-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 66243-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66243-1-Ig (CA9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66243-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66243-1-Ig (CA9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 66243-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20 ug/test Anti-Human CA9 (66243-1-Ig, Clone:1G4G5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, Hausschwein-Hirngewebe, Haussschwein-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66243-1-Ig bindet in WB, IHC, IF, FC, ELISA CA9 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CA9 fusion protein Ag1540
• Vollständiger Name: carbonic anhydrase IX
• Berechnetes Molekulargewicht: 459 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC014950
• Gene symbol: CA9
• Gene ID (NCBI): 768
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549.

Publikationen

Species	Application	Title
human	IF	Mol Oncol; Identification and validation of a combined hypoxia and immune index for triple-negative breast cancer.; Authors - Shaoquan Zheng
human	IF	Elife; Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression; Authors - Qiangqiang Liu
human	WB	Cell Rep; ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis; Authors - Fangquan Chen
human	IHC	Int J Gen Med; Identification of Hypoxia-Associated Signature in Colon Cancer to Assess Tumor Immune Microenvironment and Predict Prognosis Based on 14 Hypoxia-Associated Genes; Authors - Peng Chen
pig	IHC	J Transl Med; Tumor microenvironment in a minipig model of spinal cord glioma; Authors - Muhibullah S Tora

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (04-16-2019)

Specific band in hypoxic mouse kidney.Note: The lower part of the blot (<25 kDa) was stained for a hypoxic marker (Ndufa4l2).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, hypoxic (lane1), normoxic (lane2)

CA9 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, hypoxic (lane1), normoxic (lane2) (Cat no:66243-1-Ig)