CAB39L Polyklonaler Antikörper

CAB39L Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 16137-1-AP

Synonyme

Mo25-like protein, Mo25 like protein, MO25 BETA, Calcium-binding protein 39-like, Antigen MLAA-34

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  WB analysis of K-562 using 16137-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16137-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 16137-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 16137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16137-1-AP (CAB39L Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lymphoma using 16137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16137-1-AP (CAB39L Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 16137-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CAB39L antibody (16137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, humanes Milzgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

16137-1-AP bindet in WB, IHC, IF/ICC, ELISA CAB39L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAB39L fusion protein Ag9181
• Vollständiger Name: calcium binding protein 39-like
• Berechnetes Molekulargewicht: 337 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC010993
• Gene symbol: CAB39L
• Gene ID (NCBI): 81617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Medicina (Kaunas); Bioinformatics Prediction and Experimental Verification Identify CAB39L as a Diagnostic and Prognostic Biomarker of Kidney Renal Clear Cell Carcinoma; Authors - Yunfei Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size. No additional background bands were observed around the size range. AWESOME!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS

CAB39L Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS (Cat no:16137-1-AP)