CABYR Polyklonaler Antikörper

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• KD/KO Validated

CABYR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 12351-1-AP

Synonyme

CABYR, Calcium binding protein 86, Cancer/testis antigen 88, CBP86, CT88, Fibrousheathin 2, Fibrousheathin II, FSP 2, FSP2

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  WB analysis of human testis using 12351-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12351-1-AP (CABYR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12351-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12351-1-AP (CABYR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12351-1-AP (CABYR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12351-1-AP (CABYR Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12351-1-AP (CABYR antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human pancreas cancer using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12351-1-AP (CABYR antibody) at dilution of 1:50 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Produktinformation

12351-1-AP bindet in WB, IHC, IF, ELISA CABYR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CABYR fusion protein Ag3012
• Vollständiger Name: calcium binding tyrosine-(Y)-phosphorylation regulated
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 86-90 kDa
• GenBank-Zugangsnummer: BC011996
• Gene symbol: CABYR
• Gene ID (NCBI): 26256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	J Genet Genomics; Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice; Authors - Yaling Wang
MOUSE	WB	J Cell Sci; CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.; Authors - Samantha A M Young ; KO Validated
mouse	IF	J Cell Sci; Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice.; Authors - Ferheen Abbasi
human,mouse	IF	Clin Genet; Biallelic mutations in PMFBP1 cause acephalic spermatozoa.; Authors - Yan-Wei Sha
human	WB	Nat Commun; Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.; Authors - Ying Shen
	WB	Animals (Basel); Comparative Study on the Sperm Proteomes of Horses and Donkeys; Authors - Hong Ren