• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CACNA1A Polyklonaler Antikörper

CACNA1A Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27227-1-AP

Synonyme

APCA, BI, Brain calcium channel I, CACH4, CACN3, CACNA1A, CACNL1A4, CAV2.1, EA2, FHM, HPCA, MHP, MHP1, SCA6

Filter:
  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1A Polyclonal antibody (27227-1-AP)

    IHC staining of human brain using 27227-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27227-1-AP (CACNA1A Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1A Polyclonal antibody (27227-1-AP)

    IHC staining of human brain using 27227-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27227-1-AP (CACNA1A Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in IHChumanes Hirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27227-1-AP bindet in IHC, ELISA CACNA1A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CACNA1A fusion protein Ag25943
Vollständiger Name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Berechnetes Molekulargewicht 282 kDa
GenBank-ZugangsnummerNM_000068
Gene symbol CACNA1A
Gene ID (NCBI) 773
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IHC protocol for CACNA1A antibody 27227-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Lea (Verified Customer) (10-08-2024)

I transfected HEK 293T cells with 2 different CACNA1A constructs (12Q and 27Q, 2 replicates each) and an empty vector as a negative control. The expected band at ~280 kDa is clearly visible in the western blot and no unspecific bands are visible in the negative control. I was very happy with the results.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK 293T
CACNA1A Antibody Western Blot validation (1:5000 dilution) in HEK 293T (Cat no:27227-1-AP)
FH

Ümit (Verified Customer) (08-11-2022)

Cav2.1 (black) staining of two rhesus monkey brainstem sections (5µm &7µm) visualized with immunoperoxidase method (DAB-Nickel).

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Monkey/Purkinje Cells
CACNA1A Antibody Immunohistochemistry validation (1:100 dilution) in Monkey/Purkinje Cells (Cat no:27227-1-AP)