CACNA1B Polyklonaler Antikörper

CACNA1B Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19681-1-AP

Synonyme

BIII, Brain calcium channel III, CACH5, CACNA1B, CACNL1A5, CACNN, Cav2.2

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Galerie der Validierungsdaten

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  IHC staining of mouse brain using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19681-1-AP (CACNA1B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19681-1-AP (CACNA1B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19681-1-AP (CACNA1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19681-1-AP (CACNA1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

19681-1-AP bindet in WB, IHC, ELISA CACNA1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
• Berechnetes Molekulargewicht: 262 kDa
• GenBank-Zugangsnummer: NM_000718
• Gene symbol: CACNA1B
• Gene ID (NCBI): 774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CACNA1B, also named as CACH5, CACNL1A5 and BIII, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). CACNA1B may play a role in directed migration of immature neurons.

Publikationen

Species	Application	Title
rat	WB	Mol Neurobiol; Genomic Action of Sigma-1 Receptor Chaperone Relates to Neuropathic Pain.; Authors - Shao-Ming Wang
human	WB	Front Pharmacol; Sigma-1 Receptor Plays a Negative Modulation on N-type Calcium Channel.; Authors - Kang Zhang
human	WB	J Recept Signal Transduct Res; CACNA1B facilitates breast cancer cell growth and migration by regulating cyclin D1 and EMT: the implication of CACNA1B in breast cancer.; Authors - Yong-Mei Jin
human	WB	Cell Death Dis; Cav2.2-NFAT2-USP43 axis promotes invadopodia formation and breast cancer metastasis through cortactin stabilization; Authors - Ying Xue ; KO Validated

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