CACNA1S Polyklonaler Antikörper

CACNA1S Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22279-1-AP

Synonyme

CACH1, CACN1, CACNL1A3, Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Cav1.1

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  WB analysis using 22279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 22279-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 22279-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

22279-1-AP bindet in WB, IHC, ELISA CACNA1S und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
• Berechnetes Molekulargewicht: 212 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: NM_000069
• Gene symbol: CACNA1S
• Gene ID (NCBI): 779
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CACNA1S, also named as CACH1, CACN1 and CACNL1A3, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family and CACNA1S subfamily. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. The antibody is specific to CACNA1S.

Publikationen

Species	Application	Title
human	IHC	Reproduction; Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.; Authors - Ru Zheng
human	WB	bioRxiv; Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM; Authors - Jorge L Jimenez-Macias