CACNB3 Rekombinanter Antikörper

CACNB3 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242088E4

Kat-Nr. : 84762-4-RR

Synonyme

CACNLB3, CACNB 3, CAB3, 242088E4

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  IF Staining of MCF-7 using 84762-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CACNB3 antibody (84762-4-RR, Clone: 242088E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of SKOV-3 using 84762-4-RR

  1x10^6 SKOV-3 cells were intracellularly stained with 0.25 ug CACNB3 Recombinant antibody (84762-4-RR, Clone:242088E4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84762-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84762-4-RR against Human CACNB3 were performed. The affinity constant is 0.191 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): SKOV-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84762-4-RR bindet in IF/ICC, FC (Intra), ELISA CACNB3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CACNB3 fusion protein Ag35651
• Vollständiger Name: calcium channel, voltage-dependent, beta 3 subunit
• Berechnetes Molekulargewicht: 55kDa，484aa
• GenBank-Zugangsnummer: NM_000725.3
• Gene symbol: CACNB3
• Gene ID (NCBI): 784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CACNB3, also known as CACNLB3, is a voltage-gated Ca2+ channel subunit. It has 5 isoforms and may play a role in the regulation of transcription factors and calcium transport.