CACYBP Polyklonaler Antikörper

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• KD/KO Validated

CACYBP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11745-1-PBS

Synonyme

Calcyclin, S100 alpha 6, PNAS 107, hCacyBP, GIG5

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  WB analysis of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

  HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, and rat liver tissue were subjected to SDS PAGE followed by western blot with 11745-1-AP (CACYBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11745-1-AP (same clone as 11745-1-PBS)

  IP result of anti-CACYBP (IP:11745-1-AP, 4ug; Detection:11745-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CACYBP antibody (11745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CACYBP Polyclonal antibody (11745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11745-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA CACYBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CACYBP fusion protein Ag2340
• Vollständiger Name: calcyclin binding protein
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC022352
• Gene symbol: CACYBP
• Gene ID (NCBI): 27101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).