CACYBP Monoklonaler Antikörper

CACYBP Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C2G11

Kat-Nr. : 68161-1-PBS

Synonyme

Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5

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  WB analysis using 68161-1-Ig (same clone as 68161-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 68161-1-Ig (same clone as 68161-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68161-1-Ig (same clone as 68161-1-PBS)

  IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68161-1-Ig (same clone as 68161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68161-1-Ig (CACYBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68161-1-Ig (same clone as 68161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68161-1-Ig (CACYBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68161-1-Ig (same clone as 68161-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68161-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CACYBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CACYBP fusion protein Ag2340
• Vollständiger Name: calcyclin binding protein
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC022352
• Gene symbol: CACYBP
• Gene ID (NCBI): 27101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).