CAD Polyklonaler Antikörper

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CAD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16617-1-PBS

Synonyme

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  WB analysis of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP (CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

  IP result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 16617-1-AP (same clone as 16617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 16617-1-AP (same clone as 16617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16617-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CAD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAD fusion protein Ag9912
• Vollständiger Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Berechnetes Molekulargewicht: 2225 aa, 243 kDa
• Beobachtetes Molekulargewicht: 240 kDa
• GenBank-Zugangsnummer: BC014178
• Gene symbol: CAD
• Gene ID (NCBI): 790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).