CALCOCO1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CALCOCO1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 19843-1-AP

Synonyme

Calcium-binding and coiled-coil domain-containing protein 1, Calphoglin, Cocoa, Coiled-coil coactivator protein, PP13275

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  WB analysis using 19843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19843-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALCOCO1 antibody (19843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HT-1080.Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe, Maushirngewebe, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

19843-1-AP bindet in WB, IHC, IF/ICC, ELISA CALCOCO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CALCOCO1 fusion protein Ag13876
• Vollständiger Name: calcium binding and coiled-coil domain 1
• Berechnetes Molekulargewicht: 691 aa, 77 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC003177
• Gene symbol: CALCOCO1
• Gene ID (NCBI): 57658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Elife; Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.; Authors - Junghyun Lim ; KO Validated
mouse	WB	Cell Signal; Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.; Authors - Lisa M Cooper
mouse	WB	Nat Commun; Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer; Authors - Lucia Taraborrelli