CALCOCO1 Rekombinanter Antikörper

CALCOCO1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241127G7

Kat-Nr. : 84009-4-RR

Synonyme

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

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  WB analysis using 84009-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84009-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84009-4-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, Ramos-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84009-4-RR bindet in WB, IHC, ELISA CALCOCO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CALCOCO1 fusion protein Ag13876
• Vollständiger Name: calcium binding and coiled-coil domain 1
• Berechnetes Molekulargewicht: 691 aa, 77 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC003177
• Gene symbol: CALCOCO1
• Gene ID (NCBI): 57658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.