NDP52 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NDP52 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2F2C2

Kat-Nr. : 66401-1-Ig

Synonyme

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

---

• 

  WB analysis using 66401-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66401-1-Ig

  WB result of NDP52 antibody (66401-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDP52 transfected HeLa cells.

• 

  WB analysis of HeLa using 66401-1-Ig

  HeLa, Jurkat cells and human skeletal muscle tissue lysate were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66401-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 66401-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of Jurkat using 66401-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NDP52 antibody (66401-1-Ig, Clone: 2F2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, HeLa-Zellen, humanes Skelettmuskelgewebe, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

66401-1-Ig bindet in WB, IHC, IF/ICC, ELISA NDP52 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDP52 fusion protein Ag2866
• Vollständiger Name: calcium binding and coiled-coil domain 2
• Berechnetes Molekulargewicht: 446 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC015893
• Gene symbol: NDP52
• Gene ID (NCBI): 10241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Publikationen

Species	Application	Title
human	WB	EMBO Rep; NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.; Authors - Norihiko Furuya
human	WB	Autophagy; Copper-dependent autophagic degradation of GPX4 drives ferroptosis; Authors - Qian Xue
human,mouse	WB	mBio; The nonstructural protein 1 of respiratory syncytial virus hijacks host mitophagy as a novel mitophagy receptor to evade the type I IFN response in HEp-2 cells; Authors - Jing Cheng
mouse	IF	Immunity; The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance; Authors - Yinfeng Xu