Calumenin Polyklonaler Antikörper

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• KD/KO Validated

Calumenin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 17804-1-AP

Synonyme

CALU, Calumenin, Crocalbin, IEF SSP 9302

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  WB analysis of HEK-293 using 17804-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17804-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17804-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17804-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (17804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

17804-1-AP bindet in WB, IHC, IF/ICC, ELISA Calumenin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calumenin fusion protein Ag12073
• Vollständiger Name: calumenin
• Berechnetes Molekulargewicht: 315 aa, 37 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 57 kDa
• GenBank-Zugangsnummer: BC013383
• Gene symbol: Calumenin
• Gene ID (NCBI): 813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Oncogene; Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis.; Authors - Q Wang ; KD Validated
human	IF	Cancer Med; Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer.; Authors - Mili Zhang
pig	WB	Cell Tissue Res; Hormone-regulated PKA activity in porcine oviductal epithelial cells.; Authors - Juan Manuel Teijeiro
human	WB,IHC,IF	J Inflamm Res; Pan-Cancer Screening and Validation of CALU's Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer; Authors - Shi-Liang Chen
human	WB	Respir Res; CALU promotes lung adenocarcinoma progression by enhancing cell proliferation, migration and invasion; Authors - Yan Li