CAMK2D Rekombinanter Antikörper

CAMK2D Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1M10

Kat-Nr. : 81014-1-RR

Synonyme

CaMKII delta, EC:2.7.11.17, CaMK-II subunit delta, CaM kinase II subunit delta, Calcium/calmodulin-dependent protein kinase type II subunit delta

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  WB analysis using 81014-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81014-1-RR (CAMK2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 81014-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81014-1-RR (CAMK2D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 81014-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81014-1-RR against Human CAMK2D were performed. The affinity constant is 81.6 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maushirngewebe, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

81014-1-RR bindet in WB, IHC, ELISA CAMK2D und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CAMK2D fusion protein Ag31276
• Vollständiger Name: calcium/calmodulin-dependent protein kinase II delta
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 54-59 kDa
• GenBank-Zugangsnummer: BC032784
• Gene symbol: CAMK2D
• Gene ID (NCBI): 817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CaMKII delta, also named as CAMKD, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It may also recognize CAMK2A due to the high homology.

Publikationen

Species	Application	Title
human	WB	J Transl Med; F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration; Authors - Wuping Yang