CaMKIV Polyklonaler Antikörper

CaMKIV Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13263-1-AP

Synonyme

CaM kinase GR, CaMK GR, CAMK4, CaMKIV

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Galerie der Validierungsdaten

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  WB analysis of human cerebellum using 13263-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13263-1-AP (CaMKIV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13263-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13263-1-AP (CaMKIV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Cerebellum-Gewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

13263-1-AP bindet in WB, IHC, IF, ELISA CaMKIV und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CaMKIV fusion protein Ag3922
• Vollständiger Name: calcium/calmodulin-dependent protein kinase IV
• Berechnetes Molekulargewicht: 473 aa, 52 kDa
• Beobachtetes Molekulargewicht: 56-65 kDa
• GenBank-Zugangsnummer: BC025687
• Gene symbol: CAMK4
• Gene ID (NCBI): 814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)

Publikationen

Species	Application	Title
human	WB	Oncotarget; PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.; Authors - Chao Li
human	WB	J Invest Dermatol; The Long Noncoding RNA UCA1 Negatively Regulates Melanogenesis in Melanocytes.; Authors - Shiyao Pei
mouse	WB	Toxicol Res (Camb); Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers.; Authors - Disi Bai
rat	WB,IF	Cell Death Discov; Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV.; Authors - Qingying Tang