CAMKK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CAMKK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11549-1-PBS

Synonyme

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  WB analysis of Jurkat using 11549-1-AP (same clone as 11549-1-PBS)

  WB result of CAMKK2 antibody (11549-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMKK2 transfected Jurkat cells. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11549-1-AP (same clone as 11549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11549-1-AP (same clone as 11549-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 11549-1-AP (same clone as 11549-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

• 

  IP experiment of PC-3 using 11549-1-AP (same clone as 11549-1-PBS)

  IP result of anti-CAMKK2 (IP:11549-1-AP, 4ug; Detection:11549-1-AP 1:500) with PC-3 cells lysate 960 ug. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11549-1-AP (same clone as 11549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11549-1-AP (same clone as 11549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 11549-1-AP (same clone as 11549-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CAMKK2 antibody (11549-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11549-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CAMKK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMKK2 fusion protein Ag2093
• Vollständiger Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
• Berechnetes Molekulargewicht: 588 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC026060
• Gene symbol: CAMKK2
• Gene ID (NCBI): 10645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.