CAMKK2 Rekombinanter Antikörper

CAMKK2 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251379E8

Kat-Nr. : 82720-14-RR

Synonyme

Calcium/calmodulin-dependent protein kinase kinase 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2

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  WB analysis using 82720-14-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82720-14-RR (CAMKK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82720-14-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82720-14-RR (CAMKK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 82720-14-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82720-14-RR (CAMKK2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 82720-14-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CAMKK2 antibody (82720-14-RR, Clone: 251379E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82720-14-RR

  Biolayer interferometry (BLl) kinetic assays of 82720-14-RR against Human CAMKK2 were performed. The affinity constant is below 1 pM

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82720-14-RR bindet in WB, IHC, IF/ICC, ELISA CAMKK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CAMKK2 fusion protein Ag2093
• Vollständiger Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
• Berechnetes Molekulargewicht: 588 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC026060
• Gene symbol: CAMKK2
• Gene ID (NCBI): 10645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.