CAMKMT Polyklonaler Antikörper

CAMKMT Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27970-1-AP

Synonyme

C2orf34, CaM KMT, CAMKMT, CLNMT, FLJ23451

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  WB analysis using 27970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27970-1-AP (CAMKMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27970-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CAMKMT antibody (27970-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27970-1-AP bindet in WB, IF/ICC, ELISA CAMKMT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMKMT fusion protein Ag27559
• Vollständiger Name: chromosome 2 open reading frame 34
• Berechnetes Molekulargewicht: 323 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC053733
• Gene symbol: CAMKMT
• Gene ID (NCBI): 79823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMKMT, also named as C2orf34 and CLNMT, belongs to the class I-like SAM-binding methyltransferase superfamily and CLNMT methyltransferase family. The CAMKMT enzyme participates in cell cycle and development, regulating the production of calmodulin with three methyl lysine residues directly or indirectly. It also plays a major role in the calcium-dependent signaling pathway and posttranscriptional modifications, including Lys-115. CAMKMT is expressed mainly in the brain, liver, muscle, colon, and lung and is involved in muscle cell activities that are regulated by calcium binding (PMID: 27435482). The molecular weight of CAMKMT is 36 kDa.