CAMKV Polyklonaler Antikörper

CAMKV Polyklonal Antikörper für WB, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14788-1-PBS

Synonyme

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  WB analysis of mouse brain using 14788-1-AP (same clone as 14788-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14788-1-AP (same clone as 14788-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 14788-1-AP (same clone as 14788-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 14788-1-AP (same clone as 14788-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 14788-1-AP (same clone as 14788-1-PBS)

  IP result of anti-CAMKV (IP:14788-1-AP, 4ug; Detection:14788-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14788-1-AP (same clone as 14788-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14788-1-AP (CAMKV antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14788-1-PBS bindet in WB, IF-P, IP, Indirect ELISA CAMKV und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMKV fusion protein Ag6438
• Vollständiger Name: CaM kinase-like vesicle-associated
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-75 kDa
• GenBank-Zugangsnummer: BC000497
• Gene symbol: CAMKV
• Gene ID (NCBI): 79012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).