CAMP Polyklonaler Antikörper

CAMP Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12009-1-PBS

Synonyme

CAP18, Antibacterial peptide FALL-39, Antibacterial peptide FF-33, Antibacterial peptide KR-20, Antibacterial peptide KS-30

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  WB analysis using 12009-1-AP (same clone as 12009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12009-1-AP (CAMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12009-1-AP (CAMP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12009-1-AP (CAMP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 12009-1-AP (same clone as 12009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 12009-1-AP (same clone as 12009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CAMP antibody (12009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 12009-1-AP (same clone as 12009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CAMP antibody (12009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12009-1-AP (same clone as 12009-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAMP antibody (12009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 12009-1-AP (same clone as 12009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CAMP antibody (12009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12009-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12009-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA CAMP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMP fusion protein Ag2622
• Vollständiger Name: cathelicidin antimicrobial peptide
• Berechnetes Molekulargewicht: 170 aa, 19 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC055089
• Gene symbol: CAMP
• Gene ID (NCBI): 820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.