Calnexin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Calnexin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

313

Rezensionen (3)

Kat-Nr. : 10427-2-AP

Synonyme

calnexin, CANX, P90

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Galerie der Validierungsdaten

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  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10427-2-AP

  WB result of Calnexin antibody (10427-2-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells.

• 

  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was stained with CL594-Phalloidin (red).

• 

  IF Staining of SKOV-3 using 10427-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using Calnexin antibody (10427-2-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10427-2-AP (Calnexin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10427-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calnexin (10427-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 275 publications below
• IHC: See 3 publications below
• IF: See 46 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10427-2-AP bindet in WB, IHC, IF, FC, CoIP, ELISA Calnexin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calnexin fusion protein Ag0697
• Vollständiger Name: calnexin
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC003552
• Gene symbol: CANX
• Gene ID (NCBI): 821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Publikationen

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
human	WB	Cell Discov; A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma; Authors - Xifei Jiang
		Immunity; The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity; Authors - Tian Chen
human	WB	Nat Microbiol; Modulation of membrane phosphoinositide dynamics by the phosphatidylinositide 4-kinase activity of the Legionella LepB effector.; Authors - Na Dong
	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guo-rong (Verified Customer) (02-06-2023)

Clear bands can be detected in total cell lysates rather than in extracellular vesicles, as a good EV exclusive marker

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

Calnexin Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:10427-2-AP)

FH

Saba (Verified Customer) (03-14-2022)

The band of the antibody was so sharp and specific. The dilution I used was 1:4000 and it worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000

FH

Meliti (Verified Customer) (09-25-2019)

1:1000 dilution in 5%milk PBST O/N incubation for all antibodies depicted which all come from Proteintech 1:5000 anti rabbit HRP in 5%milk PBST for 1h

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human HEK293 WCEs and exosomes released from these cells and purified by size exclusion chromatography

Calnexin Antibody Western Blot, validation (1:1000 dilution) in Human HEK293 WCEs and exosomes released from these cells and purified by size exclusion chromatography (Cat no:10427-2-AP)

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