CAP1 Polyklonaler Antikörper

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• KD/KO Validated

CAP1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16231-1-PBS

Synonyme

Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

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  WB analysis using 16231-1-AP (same clone as 16231-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 16231-1-AP (same clone as 16231-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 16231-1-AP (same clone as 16231-1-PBS)

  IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16231-1-PBS bindet in WB, IHC, IP, Indirect ELISA CAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAP1 fusion protein Ag8876
• Vollständiger Name: CAP, adenylate cyclase-associated protein 1 (yeast)
• Berechnetes Molekulargewicht: 475 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC013963
• Gene symbol: CAP1
• Gene ID (NCBI): 10487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.