CAPG-Specific Polyklonaler Antikörper

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• KD/KO Validated

CAPG-Specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 19535-1-AP

Synonyme

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 19535-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19535-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Mausnierengewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

19535-1-AP bindet in WB, IHC,ELISA CAPG-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: capping protein (actin filament), gelsolin-like
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: NM_001747
• Gene symbol: CAPG
• Gene ID (NCBI): 822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publikationen

Species	Application	Title
human	WB	Tohoku J Exp Med; Overexpression of gelsolin-like actin-capping protein is associated with progression of lung adenocarcinoma.; Authors - Shao Fangchun F ; KD Validated