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Calpain 3 Polyklonaler Antikörper

Calpain 3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28476-1-PBS

Synonyme

CAPN3, Calcium-activated neutral proteinase 3, calpain 3, (p94), Calpain L3, Calpain p94

Filter:
  • Western Blot (WB) analysis of various lysates using Calpain 3 Polyclonal antibody (28476-1-AP)

    WB analysis using 28476-1-AP (same clone as 28476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28476-1-AP (Calpain 3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Calpain 3 Polyclonal antibody (28476-1-AP)

    IHC staining of mouse skeletal muscle using 28476-1-AP (same clone as 28476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28476-1-AP (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28476-1-PBS bindet in WB, IHC, Indirect ELISA Calpain 3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Calpain 3 fusion protein Ag29454
Vollständiger Name calpain 3, (p94)
Beobachtetes Molekulargewicht 90-105 kDa, 55-60 kDa
GenBank-ZugangsnummerBC146672
Gene symbol CAPN3
Gene ID (NCBI) 825
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).