CAPN3 Monoklonaler Antikörper

CAPN3 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12A5

Kat-Nr. : 67366-1-PBS

Synonyme

1G12A5, Calcium-activated neutral proteinase 3, Calpain 3, calpain 3, (p94), Calpain L3

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  WB analysis using 67366-1-Ig (same clone as 67366-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67366-1-Ig (CAPN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67366-1-Ig (same clone as 67366-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CAPN3 antibody (67366-1-Ig, Clone: 1G12A5 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67366-1-PBS bindet in WB, IHC, IF/ICC, ELISA CAPN3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPN3 fusion protein Ag13179
• Vollständiger Name: calpain 3, (p94)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 90-105 kDa, 55-60 kDa, 30-35 kDa
• GenBank-Zugangsnummer: BC004883
• Gene symbol: CAPN3
• Gene ID (NCBI): 825
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).