CAPN5 Polyklonaler Antikörper

CAPN5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29809-1-AP

Synonyme

calpain 5, Calpain htra 3, CAPN5, FLJ46245, HTRA3, nCL 3, NCL3, New calpain 3

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  WB analysis using 29809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29809-1-AP (CAPN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29809-1-AP (CAPN5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maus-Kolongewebe, SH-SY5Y-Zellen, SKOV-3-Zellen, Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29809-1-AP bindet in WB, ELISA CAPN5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAPN5 fusion protein Ag30479
• Vollständiger Name: calpain 5
• Berechnetes Molekulargewicht: 640 aa, 73 kDa
• Beobachtetes Molekulargewicht: 65-73 kDa
• GenBank-Zugangsnummer: BC018123
• Gene symbol: CAPN5
• Gene ID (NCBI): 726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called HTRA3.