Calpain 9 Polyklonaler Antikörper

Calpain 9 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17556-1-AP

Synonyme

Calpain 9, CAPN9, GC36, nCL 4, NCL4, New calpain 4, Protein CG36

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Galerie der Validierungsdaten

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  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17556-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 17556-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 17556-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPN9 antibody 17556-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Magengewebe, HL-60-Zellen, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Magengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17556-1-AP bindet in WB, IHC, IF, ELISA Calpain 9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calpain 9 fusion protein Ag11718
• Vollständiger Name: calpain 9
• Berechnetes Molekulargewicht: 627 aa, 72 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC027993
• Gene symbol: CAPN9
• Gene ID (NCBI): 10753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).

Publikationen

Species	Application	Title
human,mouse,rat	WB,IF	Front Pharmacol; Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis; Authors - Fang Li
human	WB,IHC	Sci Rep; Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer.; Authors - Peike Peng