CAPRIN1 Monoklonaler Antikörper

CAPRIN1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6B10F11

Kat-Nr. : 66352-1-PBS

Synonyme

6B10F11, caprin 1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1

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  WB analysis using 66352-1-Ig (same clone as 66352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (66352-1-Ig, Clone: 6B10F11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66352-1-Ig(CAPRIN1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 66352-1-Ig (same clone as 66352-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (66352-1-Ig, Clone:6B10F11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51429-1

  Cytometric bead array sample test of MP51429-1, CAPRIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66352-1-PBS. Detection antibody: 66352-2-PBS.

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  Cytometric bead array standard curve of MP51429-1

  Cytometric bead array standard curve of MP51429-1, CAPRIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66352-1-PBS. Detection antibody: 66352-2-PBS. Standard:Ag7413. Range: 1.563-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66352-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA CAPRIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPRIN1 fusion protein Ag7413
• Vollständiger Name: cell cycle associated protein 1
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC001731
• Gene symbol: CAPRIN1
• Gene ID (NCBI): 4076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.