CAPZA1 Polyklonaler Antikörper

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• KD/KO Validated

CAPZA1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11806-1-AP

Synonyme

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11806-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11806-1-AP (CAPZA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 11806-1-AP

  IP Result of anti-CAPZA1 (IP:11806-1-AP, 4ug; Detection:11806-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of human ovary tumor using 11806-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11806-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 11806-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Produktinformation

11806-1-AP bindet in WB, IP, IHC, ELISA CAPZA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAPZA1 fusion protein Ag2267
• Vollständiger Name: capping protein (actin filament) muscle Z-line, alpha 1
• Berechnetes Molekulargewicht: 286 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000144
• Gene symbol: CAPZA1
• Gene ID (NCBI): 829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Publikationen

Species	Application	Title
mouse	WB,IP	J Cell Biol; Heterodimeric capping protein is required for stereocilia length and width regulation.; Authors - Matthew R Avenarius
human	WB,IHC	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
human	WB	J Exp Clin Cancer Res; CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.; Authors - Deng Huang ; KD Validated
human	IHC	Invest Ophthalmol Vis Sci; Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.; Authors - Linge Annett A
human	WB,IHC	Int J Oncol; Prognostic value of CAPZA1 overexpression in gastric cancer.; Authors - Lee Young-Joon YJ ; KD Validated
human	WB,IHC	Melanoma Res; Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.; Authors - Sun Daxin D