CAPZA1 Polyklonaler Antikörper

CAPZA1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55145-1-AP

Synonyme

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

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Galerie der Validierungsdaten

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  WB analysis of human heart using 55145-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55145-1-AP (CAPZA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55145-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55145-1-AP (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55145-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55145-1-AP (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55145-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 55145-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 55145-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 55145-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Mausherzgewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55145-1-AP bindet in WB, IHC, ELISA CAPZA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: capping protein (actin filament) muscle Z-line, alpha 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: NM_006135
• Gene symbol: CAPZA1
• Gene ID (NCBI): 829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPZA1 belongs to the F-actin-capping protein alpha subunit family. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. The potential for CAPZ is a therapeutic target in the management of heart failure. Future research is required to determine the mechanisms by which changes in CAPZ impact myocardial function and intracellular signalling, and to develop feasible strategies that can manipulate CAPZ in the intact heart. (PMID: 15614336 ) This antibody is specific to CAPZA1.