CAPZA1 Monoklonaler Antikörper

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• KD/KO Validated

CAPZA1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

10H1F9

Kat-Nr. : 66066-1-Ig

Synonyme

capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1

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  WB analysis of HEK-293 using 66066-1-Ig

  WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells.

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  WB analysis using 66066-1-Ig

  Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66066-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66066-1-Ig (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 66066-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66066-1-Ig

  Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 66066-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CAPZA1 antibody (66066-1-Ig, Clone: 10H1F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of MCF-7 using 66066-1-Ig

  Immunofluorescent analysis of () fixed MCF-7 cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

66066-1-Ig bindet in WB, IHC, IF/ICC, ELISA CAPZA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPZA1 fusion protein Ag18841
• Vollständiger Name: capping protein (actin filament) muscle Z-line, alpha 1
• Berechnetes Molekulargewicht: 286 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC000144
• Gene symbol: CAPZA1
• Gene ID (NCBI): 829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Publikationen

Species	Application	Title
human	IF	Cancers (Basel); Critical Role for Cold Shock Protein YB-1 in Cytokinesis.; Authors - Sunali Mehta
human		bioRxiv; Bacterial ubiquitin ligase engineered for small molecule and protein target identification; Authors - James S Ye