CAPZA1 Monoklonaler Antikörper

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CAPZA1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

10H1F9

Kat-Nr. : 66066-1-PBS

Synonyme

capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1

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  WB analysis of HEK-293 using 66066-1-Ig (same clone as 66066-1-PBS)

  WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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  WB analysis using 66066-1-Ig (same clone as 66066-1-PBS)

  Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66066-1-Ig (same clone as 66066-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66066-1-Ig (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66066-1-Ig (same clone as 66066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

  Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CAPZA1 antibody (66066-1-Ig, Clone: 10H1F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66066-1-Ig (same clone as 66066-1-PBS)

  Immunofluorescent analysis of () fixed MCF-7 cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66066-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CAPZA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPZA1 fusion protein Ag18841
• Vollständiger Name: capping protein (actin filament) muscle Z-line, alpha 1
• Berechnetes Molekulargewicht: 286 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC000144
• Gene symbol: CAPZA1
• Gene ID (NCBI): 829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)