CAPZA2 Polyklonaler Antikörper

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• KD/KO Validated

CAPZA2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15948-1-AP

Synonyme

CAPPA2, CAPZ, CapZ alpha 2, CAPZA2

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• 

  WB analysis of mouse brain using 15948-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15948-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15948-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15948-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15948-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 15948-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15948-1-AP

  IP result of anti-CAPZA2 (IP:15948-1-AP, 4ug; Detection:15948-1-AP 1:800) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, MCF-7-Zellen, Mausherzgewebe, Mauslebergewebe, Maus-Eierstockgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

15948-1-AP bindet in WB, IHC, IP, ELISA CAPZA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAPZA2 fusion protein Ag8544
• Vollständiger Name: capping protein (actin filament) muscle Z-line, alpha 2
• Berechnetes Molekulargewicht: 286 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-40 kDa
• GenBank-Zugangsnummer: BC005338
• Gene symbol: CAPZA2
• Gene ID (NCBI): 830
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Elife; HIPK4 is essential for murine spermiogenesis.; Authors - J Aaron Crapster
mouse	WB	Oncotarget; Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.; Authors - Mi Jeong Kwon ; KD Validated
human	WB	Biochem J; Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.; Authors - João D Santos
mouse	WB	Eur J Cell Biol; Nuclear receptor Nur77 regulates immunomechanics of macrophages; Authors - Sanne C Lith