CAPZB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CAPZB Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 25043-1-AP

Synonyme

F-actin-capping protein subunit beta, CapZbeta, CapZ beta

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  WB analysis using 25043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25043-1-AP (CAPZB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25043-1-AP (CAPZB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25043-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25043-1-AP (CAPZB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 25043-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25043-1-AP (CAPZB antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 25043-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPZB antibody (25043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, Maushodengewebe, NIH/3T3-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25043-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CAPZB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAPZB fusion protein Ag19327
• Vollständiger Name: capping protein (actin filament) muscle Z-line, beta
• Berechnetes Molekulargewicht: 277 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-31 kDa
• GenBank-Zugangsnummer: BC109241
• Gene symbol: CAPZB
• Gene ID (NCBI): 832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.; Authors - Shumin Xia ; KD Validated
human	WB,IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
mouse	WB	Development; Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.; Authors - Xiao-Zhen Zhang
human,mouse	CoIP	Cell Rep; The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.; Authors - Xiao-Zhen Zhang
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang
human	WB	BMC Biol; The temporal association of CapZ with early endosomes regulates endosomal trafficking and viral entry into host cells; Authors - Huazhang Zhu