CARD11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CARD11 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21741-1-AP

Synonyme

BIMP3, CARD11, Carma 1, CARMA1

---

Galerie der Validierungsdaten

• 

  WB analysis using 21741-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21741-1-AP (CARD11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21741-1-AP

  WB result of CARD11 antibody (21741-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD11 transfected Raji cells.

• 

  WB analysis of mouse thymus using 21741-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21741-1-AP (CARD11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 21741-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CARD11 antibody (21741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

21741-1-AP bindet in WB, IHC, IF, ELISA CARD11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CARD11 fusion protein Ag16468
• Vollständiger Name: caspase recruitment domain family, member 11
• Berechnetes Molekulargewicht: 1154 aa, 133 kDa
• Beobachtetes Molekulargewicht: 133 kDa
• GenBank-Zugangsnummer: BC111719
• Gene symbol: CARD11
• Gene ID (NCBI): 84433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

Publikationen

Species	Application	Title
human	WB,IF	Front Immunol; Identification and Characterization of a Germline Mutation in CARD11 From a Chinese Case of B Cell Expansion With NF-κB and T Cell Anergy.; Authors - Peiwei Zhao
human	WB,IF	Front Immunol; A novel CARD11 germline mutation in a Chinese patient of B cell expansion with NF-κB and T cell anergy (BENTA) and literature review; Authors - Peiwei Zhao