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CARD11 Monoklonaler Antikörper

CARD11 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F5H6

Kat-Nr. : 68269-1-PBS

Synonyme

BIMP3, CARD11, Carma 1, CARMA1

Filter:
  • Western Blot (WB) analysis of various lysates using CARD11 Monoclonal antibody (68269-1-Ig)

    WB analysis using 68269-1-Ig (same clone as 68269-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CARD11 Monoclonal antibody (68269-1-Ig)

    WB analysis of Raji using 68269-1-Ig (same clone as 68269-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using CARD11 Monoclonal antibody (68269-1-Ig)

    IF Staining of L02 using 68269-1-Ig (same clone as 68269-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68269-1-PBS bindet in WB, IF/ICC, Indirect ELISA CARD11 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CARD11 fusion protein Ag16468
Vollständiger Name caspase recruitment domain family, member 11
Berechnetes Molekulargewicht 1154 aa, 133 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC111719
Gene symbol CARD11
Gene ID (NCBI) 84433
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.