CARD6 Polyklonaler Antikörper

CARD6 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18029-1-AP

Synonyme

CARD6, CINCIN1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 18029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18029-1-AP (CARD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18029-1-AP bindet in WB, IHC CARD6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CARD6 fusion protein Ag12595
• Vollständiger Name: caspase recruitment domain family, member 6
• Berechnetes Molekulargewicht: 1037 aa, 116 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC093825
• Gene symbol: CARD6
• Gene ID (NCBI): 84674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The caspase recruitment domain (CARD) is a homotypic protein-protein interaction module that links components of signal transduction pathways implicated in the regulation of apoptosis or adaptive or innate immunity. Although much progress has been made in assigning precise roles to most CARD-containing proteins, the functions of the 1,037-amino-acid (aa) human and 1,175-aa mouse CARD6 proteins are still unknown. CARD6 has a unique structure in that it contains the CARD at the N terminus, a glutamic acid-rich region following the CARD, and a proline-rich region at the C terminus. CARD6 also harbors a 350-aa region with similarity to upregulated gene 4 (URG4), a protein that is induced in response to hepatitis Bx antigen overexpression and exerts a positive effect on proliferation. Both CARD6 and URG4 share structural features with members of the multifaceted, IFN-inducible GTPase (IFNiGTPase) superfamily, which contains some of the proteins most abundantly induced during cell-autonomous immune responses. The calcualted molecular weight of CARD is 116 kDa, but modified CARD6 is about 130 kDa. (PMID: 18160713)

Publikationen

Species	Application	Title
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang