CARD8 Polyklonaler Antikörper

CARD8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14516-1-AP

Synonyme

DACAR, Caspase recruitment domain-containing protein 8, N-terminus, Caspase recruitment domain-containing protein 8, C-terminus, Caspase recruitment domain-containing protein 8, CARD-inhibitor of NF-kappa-B-activating ligand

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  WB analysis using 14516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14516-1-AP (CARD8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14516-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14516-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14516-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HUVEC-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14516-1-AP bindet in WB, IHC, ELISA CARD8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CARD8 fusion protein Ag5975
• Vollständiger Name: caspase recruitment domain family, member 8
• Berechnetes Molekulargewicht: 61 kDa, 49 kDa
• Beobachtetes Molekulargewicht: 61 kDa, 49 kDa
• GenBank-Zugangsnummer: BC056891
• Gene symbol: CARD8
• Gene ID (NCBI): 22900
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARD8 belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.