CARHSP1 Rekombinanter Antikörper

CARHSP1 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230489F3

Kat-Nr. : 83300-2-PBS

Synonyme

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

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  IHC staining of mouse testis using 83300-2-RR (same clone as 83300-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83300-2-RR (CARHSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CARHSP1 antibody (83300-2-RR, Clone: 230489F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-2-RR, Clone:230489F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83300-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83300-2-RR against Human CARHSP1 were performed. The affinity constant is 4.36 nM.

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Geprüfte Anwendungen

Produktinformation

83300-2-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA CARHSP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CARHSP1 fusion protein Ag2265
• Vollständiger Name: calcium regulated heat stable protein 1, 24kDa
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• GenBank-Zugangsnummer: BC003366
• Gene symbol: CARHSP1
• Gene ID (NCBI): 23589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.