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CARM1 Rekombinanter Antikörper

CARM1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242651F10

Kat-Nr. : 85146-3-PBS

Synonyme

Protein arginine N-methyltransferase 4, PRMT4, Histone-arginine methyltransferase CARM1, EC:2.1.1.319, Coactivator-associated arginine methyltransferase 1

Filter:
  • Western Blot (WB) analysis of various lysates using CARM1 Recombinant antibody (85146-3-RR)

    WB analysis using 85146-3-RR (same clone as 85146-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85146-3-RR (CARM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85146-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85146-3-PBS bindet in WB, Indirect ELISA CARM1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name coactivator-associated arginine methyltransferase 1
Berechnetes Molekulargewicht 66 kDa
Beobachtetes Molekulargewicht63-65 kDa
GenBank-ZugangsnummerNM_199141
Gene symbol CARM1
Gene ID (NCBI) 10498
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.