CARS Polyklonaler Antikörper

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• KD/KO Validated

CARS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 15296-1-AP

Synonyme

CARS1, CYSRS, Cysteine tRNA ligase, Cysteine--tRNA ligase, cytoplasmic, cysteinyl tRNA synthetase

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  WB analysis using 15296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15296-1-AP

  WB result of CARS antibody (15296-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARS transfected HeLa cells.

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  IHC staining of mouse brain using 15296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15296-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15296-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15296-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CARS antibody (15296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

15296-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CARS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CARS fusion protein Ag7546
• Vollständiger Name: cysteinyl-tRNA synthetase
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC002880
• Gene symbol: CARS
• Gene ID (NCBI): 833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
human	WB,IP	Ann Rheum Dis; Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies; Authors - Jean-Baptiste Vulsteke
mouse	WB	Nitric Oxide; H2S alleviated sepsis-induced acute kidney injury by inhibiting PERK/Bax-Bcl2 pathway; Authors - Chengqing Song