CARS Monoklonaler Antikörper

CARS Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F7E4

Kat-Nr. : 67933-1-PBS

Synonyme

CARS, CARS1, CYSRS, Cysteine tRNA ligase, cysteinyl tRNA synthetase

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  WB analysis using 67933-1-Ig (same clone as 67933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67933-1-Ig (same clone as 67933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67933-1-Ig (same clone as 67933-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67933-1-Ig (same clone as 67933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CARS antibody (67933-1-Ig, Clone: 2F7E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67933-1-PBS bindet in WB, IF/ICC, Indirect ELISA CARS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CARS fusion protein Ag7651
• Vollständiger Name: cysteinyl-tRNA synthetase
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC002880
• Gene symbol: CARS
• Gene ID (NCBI): 833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.