MLN51/CASC3 Polyklonaler Antikörper

MLN51/CASC3 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 18047-1-AP

Synonyme

BTZ, CASC3, MLN 51, MLN51, Protein barentsz, Protein CASC3

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Galerie der Validierungsdaten

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  WB analysis using 18047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18047-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 18047-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18047-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18047-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18047-1-AP

  Immunofluorescent analysis of HepG2 cells, using CASC3 antibody 18047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, MCF-7-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

18047-1-AP bindet in WB, IHC, IF,ELISA MLN51/CASC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLN51/CASC3 fusion protein Ag12694
• Vollständiger Name: cancer susceptibility candidate 3
• Berechnetes Molekulargewicht: 703 aa, 76 kDa
• Beobachtetes Molekulargewicht: 115-120 kDa
• GenBank-Zugangsnummer: BC050526
• Gene symbol: CASC3
• Gene ID (NCBI): 22794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASC3(cancer susceptibility candidate 3) is also named as MLN51 and belongs to the CASC3 family. It is induced by CSF2, and is important in the proliferation of RA fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis (RA)(PMID:17101062). It is a nucleocytoplasmic protein containing, within its aminoterminal half, a coiled-coil domain followed by two nuclear localization signals (NLSs) responsible for nuclear localization and, in its carboxyl-terminal half, a nuclear export signal(NES) that mediates cytoplasmic retention. It possesses many potential phosphorylation sites and is found to be phosphorylated in both normal and cancer-derived cells.(PMID:17652158).

Publikationen

Species	Application	Title
human	WB	Mol Cell; Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay.; Authors - Ivone G Bruno
human	WB,IF	Mol Biol Cell; Perispeckles are major assembly sites for the exon junction core complex.; Authors - Daguenet Elisabeth E