CASK Polyklonaler Antikörper

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CASK Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55277-1-PBS

Synonyme

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  WB analysis using 55277-1-AP (same clone as 55277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 55277-1-AP (same clone as 55277-1-PBS)

  WB result of CASK antibody (55277-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CASK transfected A549 cells. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 55277-1-AP (same clone as 55277-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 55277-1-AP (same clone as 55277-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 55277-1-AP (same clone as 55277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 55277-1-AP (same clone as 55277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 55277-1-AP (same clone as 55277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55277-1-PBS bindet in WB, IHC, Indirect ELISA CASK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa, 50-55 kDa
• GenBank-Zugangsnummer: NM_003688
• Gene symbol: CASK
• Gene ID (NCBI): 8573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.