Caspase 3/p17/p19 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IHC, IF, ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

214

CloneNo.

2G4B2

Rezensionen (1)

Kat-Nr. : 66470-2-Ig

Synonyme

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66470-2-Ig

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66470-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66470-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66470-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66470-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66470-2-Ig (CASP3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66470-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 3/p17/p19 antibody (66470-2-Ig, Clone: 2G4B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Mauslebergewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 196 publications below
• IHC: See 18 publications below
• IF: See 13 publications below

Produktinformation

66470-2-Ig bindet in WB, IHC, IF, ICC, ELISA Caspase 3/p17/p19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Maus, Pflanze, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 3/p17/p19 fusion protein Ag25029
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 19 kDa, 17 kDa
• GenBank-Zugangsnummer: BC016926
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Publikationen

Species	Application	Title
rat	WB,ICC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Small; Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis; Authors - Qian-Wei Luo
mouse	WB	Nat Commun; BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency; Authors - Feixia Wang
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
human	WB	Biomaterials; Autophagy-inhibiting polymer as an effective nonviral cancer gene therapy vector with inherent apoptosis-sensitizing ability.; Authors - Jiafeng Wang
human	WB	ACS Appl Mater Interfaces; Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide; Authors - Yifan Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K. (Verified Customer) (10-26-2023)

This Ab is working good for human, rat and mouse proteins samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Liver cells