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Caspase 3/P17/P19 Monoklonaler Antikörper

Caspase 3/P17/P19 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(358)

CloneNo.

2G4B2

Kat-Nr. : 66470-2-Ig

Synonyme

CASP3, Caspase3, 2G4B2, CASP 3, CASP-3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, Mausnierengewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:3000
Immunhistochemie (IHC)IHC : 1:150-1:600
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66470-2-Ig bindet in WB, IHC, IF/ICC, ELISA Caspase 3/P17/P19 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Hund, Maus, Pflanze, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Caspase 3/P17/P19 fusion protein Ag25029
Vollständiger Name caspase 3, apoptosis-related cysteine peptidase
Berechnetes Molekulargewicht 277 aa, 32 kDa
Beobachtetes Molekulargewicht 32-35 kDa, 19 kDa, 17 kDa
GenBank-ZugangsnummerBC016926
Gene symbol Caspase 3
Gene ID (NCBI) 836
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Caspase 3/P17/P19 antibody 66470-2-IgProtokoll herunterladen
IHC protocol for Caspase 3/P17/P19 antibody 66470-2-IgProtokoll herunterladenl
IF protocol for Caspase 3/P17/P19 antibody 66470-2-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Authors - Lihao Jin
rat,mouseWB,IHC

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
mouseIHC

Biomaterials

Drug-device-field integration for mitochondria-targeting dysfunction and tumor therapy by home-tailored pyroelectric nanocomposites

Authors - Zhe Liu
humanWB

Small

Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis

Authors - Qian-Wei Luo
mouseWB

Nat Commun

BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency

Authors - Feixia Wang
ratWB

Acta Biomater

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Authors - Shanshan Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

K. (Verified Customer) (10-26-2023)

This Ab is working good for human, rat and mouse proteins samples.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Liver cells