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Caspase 3/P17/P19 Monoklonaler Antikörper
Caspase 3/P17/P19 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus und mehr (5)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
2G4B2
Kat-Nr. : 66470-2-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, Mausnierengewebe, Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunhistochemie (IHC) | IHC : 1:150-1:600 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 315 publications below |
| IHC | See 30 publications below |
| IF | See 27 publications below |
Produktinformation
66470-2-Ig bindet in WB, IHC, IF/ICC, ELISA Caspase 3/P17/P19 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Hund, Maus, Pflanze, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Caspase 3/P17/P19 fusion protein Ag25029 |
| Vollständiger Name | caspase 3, apoptosis-related cysteine peptidase |
| Berechnetes Molekulargewicht | 277 aa, 32 kDa |
| Beobachtetes Molekulargewicht | 32-35 kDa, 19 kDa, 17 kDa |
| GenBank-Zugangsnummer | BC016926 |
| Gene symbol | Caspase 3 |
| Gene ID (NCBI) | 836 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Caspase 3/P17/P19 antibody 66470-2-Ig | Protokoll herunterladen |
| IHC protocol for Caspase 3/P17/P19 antibody 66470-2-Ig | Protokoll herunterladenl |
| IF protocol for Caspase 3/P17/P19 antibody 66470-2-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy | ||
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
Biomaterials Drug-device-field integration for mitochondria-targeting dysfunction and tumor therapy by home-tailored pyroelectric nanocomposites | ||
Small Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis | ||
Nat Commun BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency | ||
Acta Biomater Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH K. (Verified Customer) (10-26-2023) | This Ab is working good for human, rat and mouse proteins samples.
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