Calsequestrin 2 Rekombinanter Antikörper

Calsequestrin 2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241211A3

Kat-Nr. : 84056-6-RR

Synonyme

CASQ2, 241211A3, Calsequestrin-2

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  WB analysis using 84056-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84056-6-RR (CASQ2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 84056-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 84056-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 84056-6-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of C2C12 using 84056-6-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U2OS using 84056-6-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CASQ2 Recombinant antibody (84056-6-RR, Clone:241211A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using 84056-6-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug CASQ2 Recombinant antibody (84056-6-RR, Clone:241211A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84056-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84056-6-RR against Human CASQ2 were performed. The affinity constant is 0.251 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Mausherzgewebe, Hausschwein-Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84056-6-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Calsequestrin 2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Calsequestrin 2 fusion protein Ag33584
• Vollständiger Name: calsequestrin 2 (cardiac muscle)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46-55 kDa
• GenBank-Zugangsnummer: BC022288
• Gene symbol: Calsequestrin 2
• Gene ID (NCBI): 845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.