Calsequestrin 2 Rekombinanter Antikörper

Calsequestrin 2 Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241211A3

Kat-Nr. : CL488-84056-6

Synonyme

CASQ2, 241211A3, Calsequestrin-2

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  IF Staining of C2C12 using CL488-84056-6

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488 CASQ2 antibody (CL488-84056-6, Clone: 241211A3 ) at dilution of 1:200.

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  FC experiment of U2OS using CL488-84056-6

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Casq2 Recombinant Antibody (CL488-84056-6, Clone:241211A3)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using CL488-84056-6

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Casq2 Recombinant Antibody (CL488-84056-6, Clone:241211A3)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen, A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-84056-6 bindet in IF/ICC, FC (Intra) Calsequestrin 2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Calsequestrin 2 fusion protein Ag33584
• Vollständiger Name: calsequestrin 2 (cardiac muscle)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46-55 kDa
• GenBank-Zugangsnummer: BC022288
• Gene symbol: Calsequestrin 2
• Gene ID (NCBI): 845
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.