Caveolin-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caveolin-1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

81

Rezensionen (7)

Kat-Nr. : 16447-1-AP

Synonyme

BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 16447-1-AP

  WB result of Caveolin-1 antibody (16447-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells.

• 

  WB analysis using 16447-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16447-1-AP (Caveolin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 16447-1-AP

  IP Result of anti-Caveolin-1 (IP:16447-1-AP, 3ug; Detection:16447-1-AP 1:500) with A549 cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 67 publications below
• IHC: See 13 publications below
• IF: See 18 publications below
• IP: See 4 publications below

Produktinformation

16447-1-AP bindet in WB, IP, IHC, IF, ELISA Caveolin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caveolin-1 fusion protein Ag8049
• Vollständiger Name: caveolin 1, caveolae protein, 22kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).

Publikationen

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	WB	Nucleic Acids Res; Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis.; Authors - Huawei Wang
mouse	WB	Dev Cell; Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.; Authors - Yan Gong
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	IF	Small; Smart Carbon Nanotubes with Laser-Controlled Behavior in Gene Delivery and Therapy through a Non-Digestive Trafficking Pathway.; Authors - Fenfen Kong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (01-20-2024)

Excellent antibody for use by western blotting. Diluted in 3% BSA PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

Caveolin-1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:16447-1-AP)

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Emma (Verified Customer) (03-15-2022)

Works really well @ 1:1000 for WB in PC3 and DU145 cells. Single band seen.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

FH

Kushal (Verified Customer) (08-09-2021)

We tried to use the antibody at high concentrations of 1:100, yet we do not get bands in our blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Caco-2

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