CBFA2T2 Polyklonaler Antikörper

CBFA2T2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, chIP,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11336-1-AP

Synonyme

CBFA2T2, EHT, MTG8 like protein, MTG8 related protein 1, MTGR1, p85, Protein CBFA2T2, ZMYND3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11336-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11336-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11336-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11336-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11336-1-AP (CBFA2T2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• ChIP: See 1 publications below

Produktinformation

11336-1-AP bindet in WB, IHC, chIP,ELISA CBFA2T2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBFA2T2 fusion protein Ag1880
• Vollständiger Name: core-binding factor, runt domain, alpha subunit 2; translocated to, 2
• Berechnetes Molekulargewicht: 604 aa, 67 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC015066
• Gene symbol: CBFA2T2
• Gene ID (NCBI): 9139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Core-binding factor, runt domain, alpha subunit 2, translocated to 2(CBFA2T2) belongs to a family of ubiquitously expressed transcriptional repressors that interact with transcription factors bound to promoters of target genes and with histone deacetylases (HDACs) critical for enforcement of repressor function.CBFA2T2, also named as MTGR1 or EHT, is an 85-kDa phosphoprotein. It can bind AML1-MTG8 fusion protein in acute myeloid leukemia. The binding leads to an enhancement of cell proliferation mediated in a murine myeloid model. The calculated molecular weight of CBFA2T2 is 67 kDa, but modified SMARCE1 is about 80 kDa.

Publikationen

Species	Application	Title
	chIP	Oncogene; Myeloid translocation genes differentially regulate colorectal cancer programs.; Authors - B Parang